DHRS4 Rabbit pAb - Q9BTZ2

Core Product Specifications and Parameters

Biological Background: DHRS4 Function and Localization

• DHRS4 (Dehydrogenase/reductase SDR family member 4) is a member of the short-chain dehydrogenase/reductase (SDR) family, also known as NADPH-dependent carbonyl reductase (CR), NADPH-dependent retinol dehydrogenase/reductase (NRDR), and short chain dehydrogenase/reductase family 25C member 2 (SDR25C2).
• The primary isoform (isoform 1) functions as an NADPH-dependent oxidoreductase that catalyzes the reduction of various carbonyl compounds, including ketosteroids, α-dicarbonyl compounds, aldehydes, aromatic ketones, and quinones. It exhibits stereoselectivity in reducing 3-ketosteroids to 3β-hydroxysteroids and benzil to R-benzoin, distinct from non-primate DHRS4 enzymes. Related references: PMID:18571493 PMID:19056333
• DHRS4 participates in the metabolism of 3β-hydroxysteroids, isatin, and xenobiotic carbonyl compounds, playing a role in detoxification pathways.
• An alternatively spliced isoform (isoform 2) may lack catalytic activity due to the absence of a functional catalytic site.
• Subcellularly, DHRS4 is localized to peroxisomes and the nucleus, suggesting roles in lipid metabolism and nuclear processes.
• The protein is predominantly expressed in the liver, with additional expression observed in normal cervix and some neoplastic cervical tissues, indicating tissue-specific regulatory functions.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its enzymatic activity or subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length DHRS4 (amino acids 1–278), ensuring recognition of multiple epitopes; as a polyclonal antibody, it may detect endogenous DHRS4 across human, mouse, and rat samples in WB and ELISA applications.
• For Western blot, consider using a dilution range of 1:500–1:2000 and validate in the relevant sample system (e.g., liver or cervical tissue lysates).
• In ELISA, optimize coating antigen concentration and detection antibody dilution based on the specific assay format. Cross-reactivity with mouse and rat suggests utility for comparative studies; however, individual experimental validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying DHRS4-related xenobiotic metabolism, steroid biochemistry, or cancer biology, CamelBio can source specialized reagents and provide technical support to accelerate assay development from concept to clinic.