DHFR Rabbit mAb for WB, ELISA - P00374

Core Product Specifications and Parameters

Biological Background: DHFR Function and Localization

• DHFR (Dihydrofolate reductase) is a critical enzyme in folate metabolism, catalyzing the NADPH-dependent reduction of 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate, which is essential for de novo thymidylate, glycine, and purine synthesis, as well as DNA precursor production. Related references: PMID:12096917 PMID:15039552 PMID:17569517 PMID:21876188 PMID:22235121
• It binds its own mRNA and that of DHFR2, suggesting an autoregulatory mechanism in folate metabolism.
• DHFR localizes to the mitochondrion, cytoplasm, and nucleus, reflecting its multifunctional roles in distinct cellular compartments.
• The protein is widely expressed in fetal and adult tissues, with notably higher expression in the adult brain compared to the fetal brain.
• Alternative splicing and polymorphisms in DHFR are linked to methotrexate resistance, a key consideration in cancer chemotherapy.
• Belongs to the dihydrofolate reductase family; functions as an oxidoreductase and RNA-binding protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 88–187) of human DHFR; this sequence is largely conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band near 21 kDa; use positive and negative controls appropriate for your sample type to confirm specificity.
• In ELISA applications, consider pairing this antibody with a capture antibody targeting a different epitope for sandwich format development.
• DHFR expression can be modulated by antifolate drugs like methotrexate; account for possible fluctuations in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop partner for IVD raw materials, offering validated anti-DHFR monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents for folate metabolism research and diagnostic development. From concept to clinic, we streamline sourcing of high-quality raw materials tailored to your assay needs, including rare or custom targets.