DGCR8 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q8WYQ5

Core Product Specifications and Parameters

Biological Background: DGCR8 Function and Localization

• DGCR8, also known as DiGeorge syndrome critical region 8 or pasha (Gene symbol: C22orf12, DGCRK6), is a subunit of the microprocessor complex.
• Functions as a molecular anchor within the microprocessor complex, recognizing pri-miRNA at dsRNA-ssRNA junctions and directing DROSHA to cleave 11 bp away, producing pre-miRNA for further processing by DICER. Related references: PMID:26027739 PMID:26748718
• Its activity is heme-dependent: the heme-bound DGCR8 dimer binds pri-miRNAs cooperatively as a trimer of dimers to trigger cleavage, whereas the heme-free monomer is much less active. Related references: PMID:15531877 PMID:15574589 PMID:15589161
• Specifically recognizes N6-methyladenosine (m6A)-modified pri-miRNAs, a modification critical for efficient pri-miRNA processing. Related references: PMID:25799998
• Plays a role in DNA repair by facilitating the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks, promoting clearance of DNA damage. Related references: PMID:34188037
• Localizes to the nucleus and nucleolus.
• Ubiquitously expressed across tissues.
• Contains heme-binding, iron-binding, and RNA-binding domains; subject to phosphorylation and ubiquitination (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 180-340 of human DGCR8, a region that may confer specificity to the N-terminal portion of the protein; consider using isoform-specific controls if multiple isoforms are present.
• Validated applications include WB (predicted band ~86 kDa), IHC-P, IP, and ELISA; optimize antibody dilution for each assay and sample type.
• Predicted to cross-react with mouse and rat DGCR8; confirm reactivity in your experimental system.
• For IHC-P, appropriate antigen retrieval and blocking conditions should be empirically determined.
• Shipping on ice bags; store at -20°C upon receipt and avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical services, supporting diagnostic developers from concept to clinic. We supply validated antibodies, including this DGCR8 rabbit pAb, along with antibody pairs, bulk reagents, and customized sourcing solutions for miRNA research and related applications. Contact us for one-stop procurement of high-quality immunological reagents tailored to your assay development needs.