Desmoplakin Rabbit mAb for WB, IF-P, IHC-P, ELISA - P15924

Core Product Specifications and Parameters

Biological Background: Desmoplakin Function and Localization

• Desmoplakin is a core component of desmosomes, essential for cell–cell adhesion and epidermal barrier integrity.
  Related references: PMID:25733715, PMID:26604139
• It negatively regulates keratinocyte differentiation by modulating MAPK and PI3K pathways, as well as EGFR stability through the COP9 signalosome.
  Related references: PMID:17475244, PMID:28891468
• Desmoplakin facilitates gap junction communication by promoting microtubule-dependent trafficking of connexin-43 (GJA1) to the plasma membrane.
  Related references: PMID:25225338
• The protein binds telomere DNA and helps prevent telomere damage, cooperating with TRF2 to maintain telomere length.
  Related references: PMID:31595153
• It is localized at desmosomes, cell membranes, the cytoplasm, and the nucleus, with high expression in cardiac muscle, skin keratinocytes, and oral mucosa.
• Mutations in DSP cause arrhythmogenic cardiomyopathy and skin fragility disorders, underscoring its critical role in tissue integrity.

Experimental Guidance and Technical Tips

• Due to the large molecular weight (~332 kDa), use low-percentage acrylamide gels (e.g., 6–8%) and extended transfer times with high-molecular-weight markers for western blotting.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimize antigen retrieval and permeabilization steps; positive controls such as human skin or heart tissue are recommended.
• Validate the antibody’s performance in your specific sample type and assay conditions, as cross-reactivity has been broadly reported for human, mouse, and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials and expert sourcing services. This Desmoplakin monoclonal antibody supports studies in cell adhesion, cardiovascular biology, and dermatological diseases, and we offer a full range of complementary reagents including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary materials. For custom or rare-target raw material needs, our team provides end-to-end technical consulting and sourcing support to accelerate your project from concept to clinic.