Desmocollin 3 (DSC3) Rabbit Polyclonal Antibody for WB, ELISA - Q14574

Core Product Specifications and Parameters

Biological Background: Desmocollin-3 Function and Localization

• Desmocollin-3 (DSC3), also known as Cadherin family member 3, Desmocollin-4, or HT-CP, is a member of the desmocollin subfamily of cadherins that mediate calcium-dependent cell adhesion.
• It is a core component of desmosomes, intercellular junctions that mechanically couple cells by anchoring intermediate filaments, and is essential for maintaining epidermal cohesion and barrier function.
• DSC3 is critical for cell-cell adhesion in the basal and spinous layers of the epidermis, and its loss compromises dermal integrity (PubMed:19717567).
  Related references:
  PMID:19717567
• The protein is also implicated in telogen hair shaft anchorage and embryo compaction, reflecting its roles in both tissue architecture and early development.
• Subcellular localization is primarily at the desmosomal plaque of the cell membrane, with possible cytoplasmic pools, consistent with its dynamic junctional turnover.
• Tissue expression is prominent in stratified squamous epithelia, including the epidermis, buccal mucosa, esophagus, and cervix, with weaker expression in the granular layer (PubMed:19717567, PubMed:7665906).
  Related references:
  PMID:19717567
  PMID:7665906
• DSC3 is a glycoprotein with calcium-binding cadherin repeats, and it undergoes alternative splicing and proteolytic cleavage, features that may regulate its adhesive properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular domain (amino acids 576–690) of human DSC3; this region is conserved in rat, supporting the observed cross-reactivity.
• Predicted molecular weight is approximately 100 kDa; for Western blot, consider using a 6–10% SDS‑PAGE gel and verify the band against appropriate molecular weight markers.
• In ELISA, the recombinant protein fragment can serve as a positive control; titration of the primary antibody is recommended to optimize signal-to-noise ratio.
• Since the antibody is a polyclonal preparation, lot‑to‑lot variation is possible; include internal reference controls when comparing experimental repeats.
• Store lyophilized antibody at -20°C upon receipt; after reconstitution, aliquot and store at -20°C or -80°C to maintain stability, avoiding repeated freeze‑thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated antibody pairs and monoclonal/polyclonal antibodies for targets like Desmocollin-3. Our platform streamlines procurement through bulk ancillary reagents and custom sourcing for rare targets, supported by technical consulting from concept to clinic. Partner with CamelBio to accelerate your dermal biology and cell adhesion assay development.