Desmin Rabbit Polyclonal Antibody for WB, ELISA - P17661

Core Product Specifications and Parameters

Biological Background: Desmin Function and Localization

• Desmin is a muscle-specific type III intermediate filament protein encoded by the DES gene (aliases: CSM1, CSM2, CDCD3, LGMD1D, LGMD1E, LGMD2R), with human GeneID 1674.
• It plays a crucial role in maintaining sarcomere structure by interconnecting Z-disks, forming myofibrils, and providing mechanical strength during muscle contraction (PubMed:25358400, PubMed:24200904, PubMed:25394388, PubMed:26724190). Related references: PMID:25358400, PMID:24200904, PMID:25394388, PMID:26724190
• In adult striated muscle, desmin forms a fibrous network that connects myofibrils to each other and to the plasma membrane at the Z-line periphery (PubMed:24200904, PubMed:25394388, PubMed:26724190). Related references: PMID:24200904, PMID:25394388, PMID:26724190
• Functions as a sarcomeric microtubule-anchoring protein through specific association with detyrosinated tubulin-alpha, contributing to microtubule buckling and mechanical resistance (By similarity).
• Ensures nuclear membrane integrity by anchoring at the cell tip and nuclear envelope, helping to maintain intracellular mechanical forces (By similarity).
• Contributes to transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells and aids in maintaining nebulette conformation for cardiac alpha-actin binding (By similarity).
• Subcellular localization includes cytoplasm, myofibril sarcomere Z-line, sarcolemma, nucleus, cell tip, and nuclear envelope.
• Post-translational modifications include ADP-ribosylation, phosphorylation, and ubiquitin conjugation; disease-associated mutations are linked to cardiomyopathy, desmin-related myopathy, limb-girdle muscular dystrophy, and myofibrillar myopathy.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to amino acids 1-200 (N-terminal head domain) of human Desmin, is less conserved among intermediate filaments; consider using this antibody in Western blotting (WB) to detect full-length Desmin at ~54 kDa in human and mouse samples.
• For ELISA applications, the recombinant protein immunogen suggests direct binding capability; however, optimal coating and detection conditions should be empirically determined.
• As a polyclonal antibody, batch-to-batch variation may occur; including appropriate positive controls (e.g., Desmin-expressing muscle lysates) and negative controls (non-muscle cells) is recommended.
• For mouse cross-reactivity, validate performance in the specific mouse model or tissue because epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline IVD raw material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For Desmin-focused projects—such as muscle disease biomarker assays or sarcomeric protein interaction studies—CamelBio’s integrated supply chain and technical consulting help accelerate development from concept to clinic.