DEPDC6 Rabbit Polyclonal Antibody for WB and ELISA - Q8TB45

Core Product Specifications and Parameters

Biological Background: DEPDC6 Function and Localization

• DEPDC6 encodes DEPTOR (DEP domain-containing mTOR-interacting protein), also known as DEP domain-containing protein 6, which contains two DEP domains involved in protein-protein interactions.
• DEPTOR acts as a negative regulator of the mTORC1 and mTORC2 complexes by inhibiting the protein kinase activity of MTOR, thereby inactivating both complexes. Related references: PMID:19446321 PMID:22017875 PMID:22017876
• DEPTOR-mediated inhibition of mTORC1 and mTORC2 is required for the induction of autophagy. Related references: PMID:22017875 PMID:22017876 PMID:22017877
• In contrast to AKT1S1/PRAS40, DEPTOR only partially suppresses mTORC1 activity, suggesting a distinct regulatory mechanism. Related references: PMID:34519268 PMID:34519269
• The protein localizes to the lysosome membrane, which is consistent with its role in modulating mTOR complex signaling at the lysosomal surface.
• DEPTOR undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, and exists in several isoforms through alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–217) of human DEPDC6, which may recognize multiple isoforms; consider validating antibody specificity using siRNA or knockout controls in your target cell lines.
• For Western blot (WB), prepare whole cell lysates from human or mouse tissues/cells, and expect a band around 46 kDa; optimize blocking and antibody dilution for your specific experimental conditions.
• For ELISA, consider using recombinant DEPDC6 protein or peptide as the coating antigen, and titrate both the capture/detection antibody concentrations to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including this DEPDC6 antibody for mTOR pathway and autophagy research. Beyond polyclonal antibodies, our portfolio encompasses matched antibody pairs, bulk ancillary reagents, and rare-target sourcing capabilities, ensuring reliable supply from early R&D through clinical deployment.