DEK Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - P35659

Core Product Specifications and Parameters

Biological Background: DEK Function and Localization

• Protein DEK, also known as D6S231E, is a proto-oncogene and chromatin-associated factor implicated in DNA topology regulation.
• Functions in chromatin organization, modulating DNA supercoiling and influencing gene expression programs.
• Possesses DNA-binding activity and is classified under the DEK family of chromatin architectural proteins.
• Localizes primarily to the nucleus, consistent with its role in maintaining chromatin structure.
• Undergoes multiple post-translational modifications, including phosphorylation, acetylation, and ADP-ribosylation, which may regulate its activity.
• Ubiquitously expressed across human tissues, with relatively high abundance.
• Involved in chromosomal rearrangements; acts as a fusion partner in certain leukemias, highlighting its oncogenic potential.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 51–200 of human DEK, targeting a central region; the antibody may recognize multiple isoforms if they retain this epitope.
• For Western blotting, a band around 43 kDa is expected; consider using reducing conditions and appropriate controls.
• For IHC-P and IF/ICC, validate fixation and permeabilization protocols in the relevant sample type, as nuclear antigens may require optimized antigen retrieval.
• ELISA applications can be developed using this antibody as capture or detection reagent; pairing with another DEK antibody may enable sandwich assays.
• Cross-reactivity with rat is indicated, but confirm in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

Targeting the chromatin regulator and proto-oncogene DEK, this product addresses research in cancer biology and epigenetics. CamelBio supports diagnostic manufacturers and labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. From concept to clinic, we provide one-stop access to streamline your assay development and production workflows.