DEFB4A Rabbit pAb for IHC-P, ELISA - O15263

Core Product Specifications and Parameters

Biological Background: DEFB4A Function and Localization

• DEFB4A, also known as beta-defensin 2 (BD-2, hBD-2) or skin-antimicrobial peptide 1 (SAP1), is a secreted antimicrobial peptide belonging to the defensin family, characterized by disulfide bonds.
• Exhibits potent antimicrobial activity against Gram-negative bacteria, with moderate activity against Gram-positive bacteria; activity against P. aeruginosa is salt-sensitive, decreasing at NaCl concentrations >25 mM. Related references: PMID:10837369 PMID:9202117
• Active against the yeast Candida albicans by permeabilizing the cell membrane through targeting phosphatidylinositol 4,5-bisphosphate (PIP2), causing cell fragmentation and death. Related references: PMID:10837369 PMID:30050988 PMID:9202117
• Functions as a ligand for the chemokine receptor CCR6, inducing chemotaxis of immature dendritic cells and memory T cells, linking innate and adaptive immunity. Related references: PMID:10521347 PMID:20068036
• Highly expressed in lung epithelial cells, foreskin, and trachea, with lower expression in kidney, uterus, and salivary gland; expression is predominant in the respiratory tract epithelium.
• The mature peptide has a molecular weight of approximately 7 kDa and is secreted into the extracellular space.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length mature peptide (aa 1-64); consider this when designing validation assays and interpreting results in IHC-P or ELISA.
• Due to sequence homology, the antibody cross-reacts with mouse and rat DEFB4A; validate specificity in the relevant tissue or cell systems.
• For IHC-P, optimise antigen retrieval and antibody dilutions; suitable tissue sections include lung or skin where DEFB4A is highly expressed.
• In ELISA, include proper controls (e.g., recombinant protein standard, peptide blocking) to confirm signal specificity.
• If studying salt-sensitive antimicrobial functions, perform activity assays under low-salt conditions as high salt concentrations may impair function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a complete portfolio of IVD raw materials, including validated antibody pairs, optimised polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers studying antimicrobial peptides and innate immunity, we provide one-stop sourcing for DEFB4A antibodies and related reagents, supporting your diagnostic development from concept to clinic. Whether you need rare target raw materials or technical consulting, CamelBio bridges the gap between discovery and commercial application.