DECR2 Rabbit Polyclonal Antibody (pAb) for WB, ELISA - Q9NUI1

Core Product Specifications and Parameters

Biological Background: DECR2 Function and Localization

• DECR2 is a peroxisomal enzyme belonging to the short chain dehydrogenase/reductase (SDR) family, also known as 2,4-dienoyl-CoA reductase 2, PDCR, or SDR17C1.
• It functions as an auxiliary enzyme in the β-oxidation pathway, specifically degrading unsaturated fatty enoyl-CoA esters that contain double bonds at both even- and odd-numbered positions.
• The enzyme catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to trans-3-enoyl-CoA, a crucial step in the peroxisomal degradation of unsaturated fatty acids.
• DECR2 shows activity towards short- and medium-chain 2,4-dienoyl-CoAs, as well as docosahexaenoyl-CoA (C22:6), indicating its role in processing dietary polyunsaturated fatty acids like DHA.
• It is localized to the peroxisome, consistent with its role in fatty acid metabolism.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and exists in alternatively spliced isoforms.
• A 3D-structure has been determined, providing structural insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight of DECR2 is 31 kDa; use a positive control lysate from human, mouse, or rat cells/tissues.
• Given the polyclonal nature, consider performing a blocking peptide competition or knockdown/knockout experiment to confirm specificity.
• For ELISA, the antibody can be used as a detection reagent; pairing with a capture antibody may be required for sandwich assay development.
• Start with a standard dilution range (e.g., 1:500-1:2000 for WB) and optimize based on your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials. For targets like DECR2, involved in peroxisomal fatty acid oxidation, we offer validated antibodies, recombinant proteins, and technical support to accelerate assay development. From antibody pairs to rare target sourcing, CamelBio provides integrated solutions for your IVD needs.