DDX55 Rabbit pAb for WB, ELISA - Q8NHQ9

Core Product Specifications and Parameters

Biological Background: DDX55 Function and Localization

• DDX55, also known as DEAD box protein 55, is an ATP-dependent RNA helicase encoded by the DDX55 gene. It belongs to the DEAD-box family of helicases involved in diverse RNA metabolic processes.
• It possesses ATPase activity and plays a key role in the maturation of precursor large subunit ribosomal RNAs (rRNAs), a critical step in ribosome biogenesis. Related references: PMID:33048000
• DDX55 localizes primarily to the nucleoplasm.
• The protein contains conserved DEAD-box helicase domains for ATP binding and RNA binding.
• Post-translational modifications include phosphorylation, as indicated by phosphoproteomic data.
• Proteomics studies have identified DDX55 across various human tissues, suggesting broad expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–360 of human DDX55. For Western blotting, expect a band at ~69 kDa; consider optimizing sample preparation and antibody dilution for your model.
• This polyclonal antibody is suitable for ELISA. Users should titrate the antibody and validate assay conditions for their specific application.
• Cross-reactivity has been confirmed with human and mouse samples. For other species, perform a pilot experiment to assess reactivity.
• For best results, include appropriate positive and negative controls and validate in the relevant biological sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies for RNA metabolism targets like DDX55. We support procurement of antibody pairs, bulk ancillary reagents, and rare-target raw materials, easing your path from research to diagnostic development. Contact us to explore custom sourcing solutions for your projects.