DDX51 Rabbit pAb - Q8N8A6

Core Product Specifications and Parameters

Biological Background: DDX51 Function and Localization

• DDX51 is an ATP-dependent RNA helicase that plays a critical role in the biogenesis of the 60S ribosomal subunit.
• It belongs to the DEAD box protein family, characterized by the conserved DEAD (Asp-Glu-Ala-Asp) motif, and functions as an RNA-binding protein and hydrolase.
• DDX51 localizes predominantly to the nucleolus within the nucleus, the site of ribosomal RNA processing and ribosome assembly.
• It is predicted to bind ATP and catalyze the unwinding of RNA secondary structures using energy from ATP hydrolysis.
• Post-translational modifications include acetylation and phosphorylation, as identified through proteomics studies.
• As part of rRNA processing, DDX51 is likely involved in the maturation of ribosomal RNA precursors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 367-666, which is conserved across human, mouse, and rat DDX51, supporting the observed cross-reactivity.
• For Western blot, consider using a denaturing and reducing gel system with appropriate positive controls (e.g., nucleolar extracts) to detect the ~72 kDa band.
• ELISA may be employed for antibody titer determination or target detection; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, providing one-stop access to antibodies, antigens, and ancillary reagents. This DDX51 antibody supports research into ribosome biogenesis and related disorders. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your assay development pipeline.