DDX50 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BQ39

Core Product Specifications and Parameters

Biological Background: DDX50 Function and Localization

• DDX50 (DEAD box protein 50), also known as Gu-beta or nucleolar protein Gu2, is an ATP-dependent RNA helicase involved in pre-mRNA splicing and ribosomal RNA production. Related references: PMID:12027455

• DDX50 acts as a viral restriction factor, inhibiting replication of vaccinia virus, herpes simplex virus, Zika virus, and dengue virus during early infection. It activates NF-kappa-B and IRF3 signaling through the adapter TICAM1, leading to interferon-beta production. Related references: PMID:28181036, PMID:35215908

• The protein localizes to the nucleolus, nucleus, and cytoplasm.

• Highest expression is observed in skeletal muscle, liver, heart, placenta, and kidney.

• DDX50 contains conserved helicase ATP-binding and RNA-binding domains and is a phosphoprotein subject to ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–135 (N-terminal region) of human DDX50; confirm reactivity with full-length target in your experimental system.
• For Western blotting, a band near 83 kDa is expected; use appropriate molecular weight markers.
• The antibody is reactive with human, mouse, and rat samples, supporting multi-species applications.
• Given nucleolar localization, immunofluorescence may show nucleolar enrichment; include DAPI or nucleolar marker co-staining.
• In antiviral studies, evaluate activity in relevant cell lines and consider viral infection time courses.
• Standard ELISA and IHC-P protocols can be adapted; optimize dilution and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this Anti-DDX50 polyclonal antibody, to support research in RNA biology and antiviral responses. Our sourcing capabilities extend to validated antibody pairs, recombinant proteins, and bulk ancillary reagents for diagnostic developers. Count on CamelBio to streamline your procurement from target selection through clinical application.