DDX39A Rabbit Polyclonal Antibody for WB and ELISA - O00148

Core Product Specifications and Parameters

Biological Background: DDX39A Function and Localization

• DDX39A is an ATP-dependent RNA helicase, also known as DEAD box protein 39, nuclear RNA helicase URH49, and BAT1.
• It plays an essential role in mRNA export from the nucleus to the cytoplasm, regulating this process partly through association with ECD. Related references: PMID:33941617
• DDX39A is involved in multiple steps of RNA metabolism, including alternative splicing. Related references: PMID:33941617, PMID:38801080
• It stimulates spliceosomal U snRNA export by enhancing RNA binding of the adapter PHAX. Related references: PMID:39011894
• The protein negatively regulates type I interferon production by promoting nuclear retention of antiviral transcripts, reducing their expression. Related references: PMID:32393512
• Independently of the interferon pathway, DDX39A exerts antiviral activity against alphaviruses by binding a conserved 5' element in viral genomic RNA. Related references: PMID:37949067
• Subcellularly, DDX39A localizes to both the nucleus and cytoplasm.
• It is detected predominantly in testis, with lower levels in brain, kidney, lung, thymus, spleen, and salivary gland.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, indicating tight regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 178-427 of human DDX39A; consider this region's conservation when assessing reported cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is 49 kDa; validate band specificity using appropriate positive controls and molecular weight markers.
• In ELISA applications, optimize coating and detection conditions for rabbit polyclonal antibody binding to ensure robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. With this DDX39A antibody, we support research into RNA metabolism, nucleocytoplasmic transport, and antiviral mechanisms, offering reliable supply solutions to accelerate your diagnostic and research workflows from concept to clinic.