DDX3 Rabbit pAb for WB, IF/ICC, ELISA - O00571

Core Product Specifications and Parameters

Biological Background: DDX3 Function and Localization

• DDX3X is a multifunctional ATP-dependent RNA helicase belonging to the DEAD box family, encoded by the DDX3X gene on the X chromosome.
• It positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-dependent manner, inhibiting cell growth, and represses CDH1/E-cadherin promoter activity. Related references: PMID:16818630, PMID:18264132
• DDX3X promotes efficient translation of complex mRNAs with structured 5'-UTRs by resolving secondary structures during ribosome scanning, and enhances IRES-mediated translation independently of its helicase activity. Related references: PMID:20837705, PMID:22872150, PMID:17667941
• It acts as a viral RNA sensor, binding viral RNAs and promoting type I interferon production via the MAVS/RIG-I pathway, contributing to innate immunity. Related references: PMID:20127681, PMID:21170385, PMID:31575075
• DDX3X is exploited by viruses such as HCV, HIV-1, Zika, and Dengue to facilitate their replication, often through interactions with viral proteins. Related references: PMID:29899501, PMID:15507209, PMID:18583960
• Subcellular localization includes the cell membrane, nucleus, cytoplasm, stress granules, inflammasome, lamellipodium, and centrosome, reflecting its diverse roles.
• Widely expressed in tissues, with notable presence in testis spermatids, epidermis, and liver. Related references: PMID:15294876, PMID:16301996, PMID:16818630
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 100–200, yielding polyclonal reactivity; consider sequence homology with mouse DDX3 for cross-reactivity.
• For Western blot, expect a band at approximately 73 kDa; validate specificity using knockdown or knockout controls in relevant cell lines.
• In immunofluorescence/ICC, multiple subcellular localizations may be observed; optimize fixation and permeabilization to preserve epitope accessibility.
• For ELISA, peptide competition assays can help confirm specific binding.
• This antibody is suitable for detecting endogenous DDX3 in human and mouse samples; always verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents. This DDX3 antibody supports investigations into RNA biology, viral pathogenesis, and cell signaling. For procurement inquiries or custom sourcing of rare targets, reach out to our team.