DDX1 Rabbit mAb for WB, IHC-P, IP, ELISA - Q92499

Core Product Specifications and Parameters

Biological Background: DDX1 Function and Localization

• ATP-dependent RNA helicase able to unwind RNA-RNA and RNA-DNA duplexes; possesses ATPase activity on RNA.
• Acts as a coactivator with RELA to enhance NF-kappa-B-mediated transcription; binds cyclin CCND2 promoter.
• Binds poly(A) RNA and may be involved in mRNA 3'-end processing and polyadenylation.
• Accessory subunit of the tRNA-splicing ligase complex, directly joining spliced tRNA halves; cooperates with archease for RTCB guanylylation. Related references: PMID:24870230
• Subcellularly located in nucleus, cytoplasm, cytoplasmic granules, cytosol, and mitochondrion.
• Highest expression in tissues of neuroectodermal origin (retina, brain, spinal cord) and retinoblastoma cell lines.
• Component of a cytoplasmic sensor for viral dsRNA, triggering antiviral innate immune responses via TICAM1.
• Required for HIV-1 and coronavirus IBV replication; binds HIV-1 RRE mRNA sequence.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 441–740 within the C-terminal helicase domain, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a band near 82 kDa is expected; consider using lysates from human, mouse, or rat cell lines as positive controls.
• IHC-P applications may require antigen retrieval optimization; retinoblastoma or brain tissue sections are recommended for validation.
• In immunoprecipitation, due to DDX1’s multiple subcellular locations, lysis buffer composition and salt concentration may need adjustment.
• ELISA performance should be verified with recombinant DDX1 protein or endogenous detection in cell extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For DDX1, a key player in RNA metabolism and viral replication, CamelBio offers tailored supply solutions to support your assay development from concept to clinic. Contact us to discuss your procurement needs for DDX1 antibodies and related reagents.