DAZL Rabbit pAb - Q92904

Core Product Specifications and Parameters

Biological Background: DAZL Function and Localization

• DAZL (Deleted in azoospermia-like) is an autosomal homolog of the Y-chromosomal DAZ gene family, also known as DAZH, DAZL1, DAZLA, or SPGYLA.
• It encodes an RNA-binding protein that is essential for gametogenesis in both males and females, with a central role in spermatogenesis.
• DAZL binds to the 3′-UTR of target mRNAs, specifically recognizing GUU triplets, to regulate their translation (by similarity).
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in mRNA transport and translation control.
• Expression is testis-specific, highlighting its critical function in germ cell development.
• Post-translational modifications include phosphorylation (phosphoprotein), and the gene undergoes alternative splicing to produce multiple isoforms.
• Keywords associated with DAZL include developmental protein, differentiation, spermatogenesis, and translation regulation, underscoring its role in reproductive biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 200-250), which may be conserved across species, consistent with reported mouse and rat cross-reactivity; verify sequence alignment if using in other species.
• For western blotting, a band at ~33 kDa is expected; however, post-translational modifications or isoforms may produce additional bands.
• In immunofluorescence and immunohistochemistry, nuclear and cytoplasmic localization should be observed in testis tissue; include appropriate controls.
• As a polyclonal antibody, lot-to-lot variability is possible; consider titrating antibody concentration for each application.
• For ELISA, the recombinant protein immunogen can serve as a positive control to assess binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this DAZL antibody, supporting reproductive health and fertility research. Our sourcing network covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and procurement of rare-target raw materials to accelerate your assay development from concept to clinic.