DAP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51397

Core Product Specifications and Parameters

Biological Background: DAP Function and Localization

• Death-associated protein 1 (DAP/DAP1) is a small (11 kDa) protein encoded by the DAP gene in humans.
• Functions as a ribosome-binding protein involved in ribosome hibernation, stabilizing ribosomes in an inactive state and preventing proteasomal degradation (By similarity).
• Associates with eIF5a at the polypeptide exit tunnel of the ribosome to prevent mRNA translation, particularly in mature oocytes where ribosome inactivation is critical for early development (By similarity).
• Acts as a negative regulator of autophagy, a cellular degradation pathway. Related references: PMID:20537536
• Mediates interferon-gamma-induced cell death, linking to apoptotic pathways. Related references: PMID:7828849
• Post-translationally modified by acetylation and phosphorylation, as identified by proteomic studies.

Experimental Guidance and Technical Tips

• For Western blot, note the small predicted molecular weight of DAP1 (11 kDa); consider using a high-percentage gel and optimized transfer conditions to retain the protein.
• Validate antibody specificity in your sample system using positive and negative controls, especially when assaying tissues with low DAP expression.
• In immunohistochemistry, antigen retrieval may be necessary as the immunogen corresponds to a linear N-terminal epitope (amino acids 1-102).
• For immunocytochemistry, fixation and permeabilization conditions should be tailored to your cell type to ensure accessibility of the ribosomal target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-DAP antibody supports research into apoptosis, autophagy, and translational regulation, and we provide fully customizable procurement solutions to accelerate your diagnostic development.