Cytokeratin 20 (CK20) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P35900

Core Product Specifications and Parameters

Biological Background: Cytokeratin 20 (CK20) Function and Localization

• Cytokeratin 20 (CK20), also designated Keratin-20 or Protein IT, is a type I cytoskeletal keratin encoded by the KRT20 gene.
• Plays a significant role in maintaining keratin filament organization in intestinal epithelia; upon phosphorylation, it contributes to mucin secretion in the small intestine (By similarity).
• As a member of the intermediate filament family, CK20 contains a coiled-coil domain and is annotated with keywords including apoptosis, phosphoprotein, and direct protein sequencing.
• Predominantly expressed in the intestinal epithelium (luminal cells of colonic mucosa) and in Merkel cells of the oral cavity (gingival, palatal, and lingual mucosa).
• Subcellular localization is cytoplasmic, consistent with its structural role.
• Due to its tissue-restricted expression pattern, CK20 is widely used as an immunohistochemical marker for gastrointestinal epithelium and Merkel cell-derived tumors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the C‑terminal region (aa 325–424) of human CK20, a domain that is unique and well‑suited for generating specific antibodies.
• For Western blotting, a band at ~48 kDa is expected; consider using intestinal or colon tissue lysates as a positive control.
• For IHC‑P, heat‑induced epitope retrieval (e.g., citrate buffer, pH 6.0) may improve detection; validate expression in normal intestinal or Merkel cell‑containing tissues and use isotype controls.
• It is recommended to titrate the antibody for each application and include both positive and negative control lysates/tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with a streamlined route to IVD raw materials, including this CK20 monoclonal antibody and its matching antigen. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and sourcing for rare‑target raw materials—supporting your development from early research through clinical application.