Cytokeratin 18 (KRT18) Rabbit Monoclonal Antibody for WB - P05783

Core Product Specifications and Parameters

Biological Background: Keratin 18 Function and Localization

• As a type I keratin, KRT18 forms obligate heteropolymers with KRT8, providing mechanical stability and resilience to simple epithelial cells. Related references: PMID:15529338, PMID:26823019

• KRT8/KRT18 filaments mediate ARHGEF40-dependent actin stress fiber formation and tensional force-induced reinforcement. Related references: PMID:26823019

• Organization and orientation of KRT8/KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity).

• Phosphorylation of KRT18 regulates intermediate filament reorganization; it also undergoes post-translational modifications such as acetylation, methylation, glycation, and ubiquitination. Related references: PMID:7523419, PMID:8522591, PMID:9298992

• KRT18 plays a role in the delivery of mutated CFTR to the plasma membrane. Related references: PMID:15529338, PMID:16424149

• It is involved in interleukin-6 (IL-6)-mediated barrier protection. Related references: PMID:17213200

• Subcellular localization includes cytoplasm, perinuclear region, nucleus, nucleolus, and nuclear matrix.

• Tissue expression: present in colon, placenta, liver, and weakly in exocervix; elevated in lymph nodes of breast carcinoma.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 250-430 of human KRT18, a region conserved across human, mouse, and rat, supporting the observed broad cross-reactivity.
• For Western blot, the predicted band is at ~48 kDa; validate using appropriate positive and negative controls.
• For IHC-P, consider antigen retrieval optimization and appropriate tissue controls; expression is prominent in simple epithelial tissues.
• For ELISA, the antibody may be used as a capture or detection reagent; pair it with a suitable secondary antibody and validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials, technical services, and consulting. This anti-KRT18 monoclonal antibody exemplifies our portfolio of validated antibodies for epithelial biology and oncology research. Beyond individual reagents, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting your entire workflow from concept to clinic.