Cytokeratin 17 (KRT17) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q04695

Core Product Specifications and Parameters

Biological Background: Cytokeratin 17 (KRT17) Function and Localization

• Cytokeratin 17 (KRT17), also known as 39.1, CK-17, or K17, is a type I keratin intermediate filament protein encoded by the KRT17 gene in humans.
• It plays a critical role in the formation and maintenance of skin appendages, particularly in hair follicle growth and cycling, helping to sustain the anagen phase.
• KRT17 modulates TNF-alpha signaling in hair cycling and regulates epithelial cell growth and protein synthesis by binding to SFN (14-3-3 sigma) and activating the Akt/mTOR pathway.
• As a cytoplasmic protein, it contributes to the structural integrity of epithelial cells and is a marker of basal cell differentiation in complex epithelia, indicating a subset of epithelial stem cells.
• The protein is involved in tissue repair and acts as a promoter of epithelial proliferation by regulating immune responses, specifically promoting a Th1/Th17-dominated immune environment in skin.
• KRT17 may serve as an autoantigen in psoriasis, with certain peptide regions triggering autoreactive T-cell proliferation, implicating it in the immunopathogenesis of this disease.
• Expression is prominent in the outer root sheath and medulla of hair follicles, nail matrix, mucosal stratified squamous epithelia, basal cells of oral epithelium, sweat and mammary glands, and palmoplantar epidermis.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitination; mutations are linked to pachyonychia congenita and steatocystoma multiplex.
• The molecular weight of KRT17 is approximately 48 kDa (48106 Da as per UniProt).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 133–432 of human KRT17, covering a large portion of the protein; this design may confer high specificity and strong cross-reactivity with mouse and rat. However, validation in the relevant sample system is recommended.
• For Western blot (WB), expect a band near 48 kDa; include appropriate positive controls (e.g., epithelial cell lysates) and loading controls to confirm specificity.
• For immunofluorescence/immunocytochemistry (IF/ICC), test a range of dilutions (e.g., 1:50–1:200) on fixed/permeabilized cells or tissue sections known to express KRT17, such as skin or mucosal epithelia, with proper negative controls.
• When using this antibody in ELISA, consider pairing it with a compatible detection antibody for sandwich assays; validate sensitivity and specificity using recombinant KRT17 protein or characterized positive samples.
• Given the role of KRT17 in psoriasis and other epithelial disorders, this antibody may be a useful tool for dermatological research; verify its performance in disease-relevant models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw-material sourcing, offering a wide range of validated antibodies—including optimized polyclonal and monoclonal antibodies like this anti-KRT17 antibody—to accelerate your epithelial biology and dermatology research. We also provide bulk ancillary reagents, technical services, and consultation to streamline your diagnostic development from concept to clinic.