Cytokeratin 17 (KRT17) Rabbit Monoclonal Antibody for WB, ELISA - Q04695

Core Product Specifications and Parameters

Biological Background: Cytokeratin 17 (KRT17) Function and Localization

• Cytokeratin 17 (KRT17) is a type I keratin intermediate filament protein, also known as 39.1, CK-17, or K17.
• It plays a key role in the formation and maintenance of skin appendages such as hair follicles, influencing their shape, orientation, and growth state (anagen phase) (By similarity).
• KRT17 modulates TNF signaling and regulates protein synthesis and epithelial cell growth via SFN binding and Akt/mTOR pathway activation (By similarity).
• In tissue repair, KRT17 acts as a promoter of epithelial proliferation and may be a marker of basal cell differentiation in complex epithelia (By similarity).
• It is involved in immune regulation in skin, promoting a Th1/Th17 immune environment and potentially contributing to basaloid skin tumors (By similarity).
• Subcellularly, KRT17 localizes to the cytoplasm.
• The protein is highly expressed in the outer root sheath and medulla of hair follicles (eyebrow, beard), nail matrix, and certain mucosal and glandular epithelia (at protein level).
• KRT17 contains a coiled-coil domain, undergoes phosphorylation, and is implicated in ectodermal dysplasia and palmoplantar keratoderma when mutated.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 332-432 of human KRT17 (NP_000413.1), which lies within a region conserved between human and mouse, supporting the reported cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 48 kDa; consider using appropriate gel percentages (e.g., 10% SDS-PAGE) and validate detection in positive control lysates such as HaCaT or HeLa cells.
• For ELISA, direct coating with the immunogen peptide or recombinant KRT17 protein may serve as a positive control; experimental determination of optimal antibody dilution is recommended.
• As a rabbit monoclonal antibody, it offers consistent lot-to-lot reproducibility; however, we recommend verifying performance in your specific sample matrix and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research institutes with validated antibodies, bulk ancillary reagents, and rare target raw-material solutions. The Cytokeratin 17 (KRT17) Rabbit mAb supports research in dermatology, oncology, and epithelial biology, complementing our portfolio of optimized panels for skin-related disorders. From monoclonal antibodies to peptide immunogens, CamelBio streamlines your procurement with technical support and supply chain efficiency.