Cytokeratin 14 (KRT14) Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P02533

Core Product Specifications and Parameters

Biological Background: Cytokeratin 14 (KRT14) Function and Localization

• Keratin 14 (KRT14) is a member of the type I keratin family, also known as cytokeratin-14 (CK-14) or keratin-14 (K14), and serves as a structural protein in epithelial cells.
• It forms obligate heteropolymers with the type II keratin KRT5, contributing to the keratin intermediate filament network that provides mechanical strength and structural integrity to the basal keratinocytes of stratified epithelia (PubMed:11724817, PubMed:1694855, PubMed:1720261). Related references: PMID:11724817, PMID:1694855, PMID:1720261
• The nonhelical tail domain of KRT14 promotes KRT5-KRT14 filaments to self-organize into large bundles, enhancing the mechanical resilience of keratin intermediate filaments in vitro (PubMed:11724817). Related references: PMID:11724817
• KRT14 is predominantly localized to the cytoplasm, but can also be found in the nucleus.
• It is expressed in the corneal epithelium and is detected in the basal layer of the epidermis, with reduced expression in the more apically located layers (stratum spinosum, stratum granulosum) and absence in the stratum corneum. It is also strongly expressed in the outer root sheath of anagen hair follicles.
• Post-translational modifications include phosphorylation and ubiquitination, and mutations in KRT14 are associated with several skin fragility disorders such as epidermolysis bullosa simplex and ectodermal dysplasia.

Experimental Guidance and Technical Tips

• The immunogen targets a synthetic peptide within amino acids 373-472, a region in the C-terminal tail domain of KRT14 that may confer specificity for the monomeric or filament-associated form of the protein.
• Western blotting detects a band at approximately 52 kDa; consider running a positive control lysate from basal keratinocyte-rich tissues (e.g., skin, cornea) to confirm specificity.
• For immunofluorescence and immunohistochemistry, both cytoplasmic and nuclear staining may be observed; validate in relevant tissue sections with appropriate antigen retrieval.
• Cross-reactivity with mouse and rat enables studies across species; adjust antibody dilutions and detection conditions for each model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. For Cytokeratin 14 (KRT14) research and epithelial marker development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio includes rare target raw-material sourcing and technical support to accelerate your assay development from concept to clinic.