CYP7A1 Mouse Monoclonal Antibody for WB, ELISA - P22680

Core Product Specifications and Parameters

Biological Background: CYP7A1 Function and Localization

• Cytochrome P450 7A1 (CYP7A1), also known as cholesterol 7-alpha-monooxygenase or CYP7, is a key hepatic enzyme initiating the classical pathway of bile acid biosynthesis.
• Catalyzes the 7-alpha hydroxylation of cholesterol, the rate-limiting step in the conversion of cholesterol to primary bile acids, thereby regulating cholesterol homeostasis. Related references: PMID:12077124, PMID:19965590, PMID:2384150
• Also hydroxylates oxysterols such as 4beta-hydroxycholesterol and 24-hydroxycholesterol, contributing to alternative bile acid synthesis and oxysterol metabolism. Related references: PMID:11013305, PMID:12077124
• Functions as a monooxygenase that uses molecular oxygen and NADPH via cytochrome P450 reductase to incorporate one oxygen atom into the substrate, while reducing the second to water. Related references: PMID:11013305, PMID:12077124, PMID:19965590
• Oxidizes the 7,8 double bond of 7-dehydrocholesterol and lathosterol, predominantly generating 7-keto derivatives, expanding its role in sterol metabolism. Related references: PMID:21813643
• Localized to the endoplasmic reticulum membrane and microsome membrane, consistent with its function as a membrane-bound cytochrome P450.
• Contains a heme iron cofactor required for its catalytic activity and is characterized by a conserved P450 structural fold.
• Primarily expressed in the liver, as detected by tissue-specific analysis, aligning with its role in hepatic cholesterol catabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal region (aa 86-108) that may be conserved across species, consistent with the observed cross-reactivity with mouse and rat CYP7A1.
• For Western blot, a specific band at approximately 58 kDa is expected. Validate with appropriate positive and negative controls, such as human liver lysates.
• ELISA applications may benefit from using recombinant CYP7A1 protein as a quantitative standard; assay conditions should be optimized empirically.
• As CYP7A1 expression is liver-specific, sample preparation should consider tissue origin; liver extracts are recommended as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this CYP7A1 mAb, which targets a key enzyme in cholesterol metabolism and liver function. We offer tailored sourcing for bulk ancillary reagents and rare targets, enabling seamless integration from discovery to clinical assay development. Let us be your trusted partner in advancing liver disease diagnostics and metabolic research.