CYP24A1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q07973

Core Product Specifications and Parameters

Biological Background: CYP24A1 Function and Localization

• CYP24A1, also known as 1,25-dihydroxyvitamin D(3) 24-hydroxylase, CP24, HCAI, CYP24, HCINF1, or P450-CC24, is a mitochondrial cytochrome P450 monooxygenase encoded by the CYP24A1 gene (Gene ID: 1591).
• It plays a central role in vitamin D catabolism and calcium homeostasis by inactivating calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)) through C24- and C23-oxidation pathways. Related references: PMID:11012668 PMID:15574355 PMID:16617161
• Through the C24-oxidation pathway, it sequentially oxidizes calcitriol to the biliary metabolite calcitroic acid. Related references: PMID:15574355 PMID:24893882
• Through the C23-oxidation pathway, it converts calcidiol to 25(OH)D3-26,23-lactone. Related references: PMID:11012668 PMID:8679605
• It also preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Related references: PMID:25727742
• Subcellular localization is mitochondrial, consistent with its role in vitamin D processing.
• Additional keywords: heme, iron, alternative splicing, disease variant, monooxygenase, oxidoreductase, transit peptide.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 36-448 of human CYP24A1, covering most of the protein; the polyclonal nature may result in recognition of multiple epitopes.
• For WB, a band around 59 kDa (theoretical 58.9 kDa) is expected; verify expression levels in your sample system.
• For IHC-P, consider optimizing antigen retrieval and include appropriate controls.
• Validate cross-reactivity in human, mouse, and rat tissues; other species may not be recognized.
• This antibody is specified for WB, IHC-P, and ELISA; further application-specific validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. This CYP24A1 antibody is ideal for vitamin D metabolism and calcium homeostasis studies. Our rare-target sourcing expertise ensures consistent supply for your assay development projects from concept to clinic.