CYP24A1 Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q07973

Core Product Specifications and Parameters

Biological Background: CYP24A1 Function and Localization

• CYP24A1 is a mitochondrial cytochrome P450 monooxygenase with a pivotal role in vitamin D catabolism and calcium homeostasis.
• Catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D3) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D3) through C24- and C23-oxidation pathways.
• Via the C24-oxidation pathway, performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. Related references: PMID:15574355, PMID:24893882
• Through the C23-oxidation pathway, catalyzes sequential oxidation of calcidiol resulting in the end product 25(OH)D3-26,23-lactone. Related references: PMID:11012668, PMID:8679605
• Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Related references: PMID:25727742
• Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. Related references: PMID:8679605
• Localized to the mitochondrion; contains a heme iron cofactor and is subject to alternative splicing. Variants have been associated with disease.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 36–410) covers a large portion of CYP24A1, suggesting potential recognition of multiple epitopes; validate in the relevant sample system for each application.
• For IHC-P, consider standard antigen retrieval methods and validate using positive control tissues known to express CYP24A1.
• In IF/ICC, a mitochondrial staining pattern is expected; confirm co-localization with mitochondrial markers if needed.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a different clone is recommended for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers focused on vitamin D metabolism, CamelBio provides reliable access to antibodies targeting key enzymes like CYP24A1, along with customized sourcing for rare and niche targets. Explore our catalog to streamline your diagnostic development from concept to clinic.