CYP11A1 Rabbit Polyclonal Antibody (WB, IF, IHC, ELISA) - P05108

Core Product Specifications and Parameters

Biological Background: CYP11A1 Function and Localization

• CYP11A1, also known as cholesterol side-chain cleavage enzyme (P450scc), is a mitochondrial cytochrome P450 monooxygenase that catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the biosynthesis of all steroid hormones. Related references: PMID:21636783
• The enzyme carries out three sequential oxidation reactions at C22 and C20 of cholesterol, ultimately cleaving the side chain to produce pregnenolone and 4-methylpentanal. Related references: PMID:21636783
• CYP11A1 is located on the mitochondrial inner membrane and requires electron transfer from NADPH via adrenodoxin reductase and adrenodoxin for its catalytic activity.
• Alternative splicing of the CYP11A1 gene produces multiple transcript variants, though the functional significance of these isoforms is not fully characterized.
• Mutations in CYP11A1 are associated with congenital adrenal hyperplasia and disorders of steroidogenesis, highlighting its critical role in endocrine function.
• The protein is a heme-iron containing monooxygenase and a member of the cytochrome P450 superfamily, with a molecular weight of approximately 60 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1-100) of human CYP11A1. This region is predicted to be part of the mature protein after mitochondrial import, potentially enabling detection of the processed form.
• For Western blot analysis, a predominant band around 60 kDa is expected; minor bands may represent isoforms or processing intermediates. Validation in the relevant tissue or cell lysate is recommended.
• In immunofluorescence and immunohistochemistry, mitochondrial staining patterns consistent with the inner membrane localization should be observed. Co-staining with organelle markers can aid interpretation.
• This polyclonal antibody has been reported to work in ELISA; however, optimal working dilutions for each application should be determined empirically in the end user's system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD manufacturers, providing one-stop access to high-quality raw materials, technical support, and consultancy from concept to clinic. This CYP11A1 antibody supports research and assay development in steroidogenesis and endocrine diagnostics, areas where CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your product pipeline.