Cyclin E1 Rabbit PolymAb® for WB and ELISA - P24864

Core Product Specifications and Parameters

Biological Background: Cyclin E1 Function and Localization

• G1/S-specific cyclin-E1 is a member of the cyclin family and is encoded by the CCNE1 gene.
• It plays an essential role in cell cycle control, specifically regulating the G1/S transition (start).
• The protein localizes predominantly to the nucleus, consistent with its function in cell cycle regulation.
• Cyclin E1 undergoes alternative splicing and post-translational modifications, including phosphorylation and ubiquitin conjugation.
• Tissue expression is highest in testis and placenta, with lower levels observed in bronchial epithelial cells.
• As a cyclin, it partners with cyclin-dependent kinases to drive cell cycle progression, and its dysregulation is associated with oncogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 300–400 of human Cyclin E1 (NP_001229.1). Consider this epitope region when designing complementary detection reagents.
• For Western blotting, the predicted molecular weight is ~47 kDa; validate using appropriate positive controls (e.g., testis or placenta lysates) and account for possible post-translational modifications.
• For ELISA, optimize the coating concentration and detection system based on the specific assay format; the antibody can serve as a capture or detection agent.
• Cross-reactivity with mouse and rat is reported; verify performance in your experimental system, as sequence homology may influence binding.
• Since the antibody is a recombinant rabbit monoclonal (PolymAb®), expect high lot-to-lot consistency and minimal background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our Cyclin E1 antibody supports oncology and cell cycle research applications, and we offer comprehensive sourcing services for rare targets, customized development, and regulatory support—bridging concepts to clinical implementation efficiently.