Cyclin D3 Rabbit mAb - P30281

Core Product Specifications and Parameters

Biological Background: Cyclin D3 Function and Localization

• Cyclin D3 is a key cell cycle regulator that forms a complex with CDK4 to phosphorylate RB1, leading to E2F release and G1/S progression. Related references: PMID:8114739
• The cyclin D3-CDK4 complex acts as a major integrator of mitogenic and antimitogenic signals, hypophosphorylating RB1 in early G1. Related references: PMID:8114739
• Nuclear translocation and activity of the cyclin D3-CDK4 complex require the ternary complex with CDKN1B (p27). Related references: PMID:16782892
• Cyclin D3 also exhibits CDK4-independent transcriptional coactivator activity with ATF5. Related references: PMID:15358120
• Subcellular localization: Predominantly nuclear but also found in the cytoplasm.
• The protein undergoes alternative splicing, phosphorylation, and ubiquitin conjugation, reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 202–292 of human Cyclin D3 (P30281); this region may confer target specificity. Confirm reactivity in your intended application.
• For western blotting, a band around 33 kDa is expected; run appropriate positive controls (e.g., cell lines expressing Cyclin D3).
• For ELISA, consider direct or sandwich formats and validate with recombinant protein or cell lysates.
• Cross-reactivity has been observed with human, mouse, and rat samples; however, validation in the actual sample system is recommended.

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CamelBio provides a comprehensive portfolio of IVD raw materials, including this cyclin D3 rabbit monoclonal antibody for cell cycle research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to support your diagnostic development. Our procurement network extends to rare targets, ensuring reliable supply for your projects.