Cyclin B1 Rabbit pAb for WB, IHC-P, IF/ICC, IP, ELISA - P14635

Core Product Specifications and Parameters

Biological Background: Cyclin B1 Function and Localization

• Cyclin B1 (encoded by CCNB1) is a key regulatory protein essential for the control of the cell cycle at the G2/M (mitosis) transition. Related references: PMID:27030811 PMID:17495531 PMID:17495533
• It binds and activates the cyclin-dependent protein kinase CDK1/CDC2, forming the mitosis-promoting factor (MPF) complex that drives mitotic entry. Related references: PMID:41100585
• Subcellular localization is dynamic: Cyclin B1 shuttles between the cytoplasm and nucleus, and associates with the microtubule organizing center (centrosome) and cytoskeleton.
• Keywords include 3D-structure, Acetylation, Alternative splicing, Cell cycle, Cell division, Cyclin, Cytoplasm, Cytoskeleton, Mitosis, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Ubl conjugation, highlighting its regulated expression and post-translational modifications.
• The protein has a predicted molecular weight of ~48 kDa (UniProt: 48337 Da) and undergoes alternative splicing to produce multiple isoforms.
• Cyclin B1 is widely expressed in proliferating cells and is commonly used as a marker for cell cycle phase analysis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 334-433 of human Cyclin B1 (NP_114172.1); this region may confer specific epitope recognition. Consider verifying cross-reactivity with intended species by sequence alignment.
• For Western blot (WB), a band around 48 kDa is expected in lysates from proliferating cells (e.g., HeLa, Jurkat). Validate the antibody in your model system to confirm specificity.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), Cyclin B1 typically shows cytoplasmic and nuclear staining patterns, with centrosomal localization in mitotic cells. Optimize antigen retrieval and permeabilization conditions.
• For immunoprecipitation (IP) and ELISA, the antibody may be suitable for capturing or detecting Cyclin B1; confirm binding efficiency using recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cell cycle research targets like Cyclin B1, we offer sourcing solutions from early-stage development to clinical applications. Our supply chain covers rare target raw materials to accelerate your assay development.