Cyclin B1 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - P14635

Core Product Specifications and Parameters

Biological Background: Cyclin B1 Function and Localization

• Essential for the control of the cell cycle at the G2/M (mitosis) transition. Related references: PMID:27030811, PMID:17495531, PMID:17495533
• Binds and activates cyclin-dependent protein kinase CDK1/CDC2 to promote mitotic entry. Related references: PMID:41100585
• The protein localizes dynamically: primarily cytoplasmic during interphase, then translocates to the nucleus at prophase, and also associates with the centrosome and microtubule organizing center.
• Cyclin B1 undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its stability, localization, and activity.
• Alternative splicing produces isoforms that may have distinct functions or regulatory roles.
• It belongs to the cyclin family, characterized by a conserved cyclin box domain that mediates CDK binding and activation.
• Cyclin B1 degradation via the ubiquitin-proteasome pathway is required for mitotic exit and progression to G1 phase.

Experimental Guidance and Technical Tips

• For western blotting, a band at approximately 48 kDa may be observed; adjust gel percentage and transfer conditions as needed.
• When performing immunoprecipitation, use a lysis buffer that preserves protein-protein interactions and include appropriate negative controls.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may enhance detection.
• In ELISA, consider pairing this clone with a suitable detection antibody or using it as a capture agent, optimizing coating concentrations.
• Because the immunogen targets amino acids 334–433 in the C-terminal region, the antibody may recognize both full-length and some truncated isoforms; validate in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We support cell cycle research with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us for customized solutions and reliable supply of Cyclin B1 antibodies and related reagents.