CXCR3-B Rabbit pAb - P49682-2

Core Product Specifications and Parameters

Biological Background: CXCR3-B Function and Localization

• CXCR3-B is isoform 2 of the C-X-C chemokine receptor type 3 (CXCR3), also known by alternative names including G protein-coupled receptor 9 (GPR9), CKR-L2, CD183, and interferon-inducible protein 10 receptor (IP-10 receptor).
• It functions as a receptor for the chemokine CXCL4 and mediates the inhibitory activities of CXCL9, CXCL10, and CXCL11 on human microvascular endothelial cell (HMVEC) proliferation, survival, and angiogenesis through a cAMP-mediated signaling pathway. Unlike the main CXCR3-A isoform, CXCR3-B does not promote cell chemotaxis. Related references: PMID:12782716
• Interaction of CXCR3-B with CXCL4 or CXCL10 leads to activation of the p38 MAPK pathway, which contributes to the inhibition of angiogenesis.
• Overexpression of CXCR3-B in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis, suggesting a tumor-suppressive role.
• The receptor is localized at the cell membrane.
• As a member of the G protein-coupled receptor family and the CXC chemokine receptor subfamily, CXCR3-B plays a distinctive role in regulating vascular biology and immune responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1–100) of human CXCR3-B, which is unique to this isoform; thus, the antibody may distinguish CXCR3-B from other splice variants. Consider confirming specificity using isoform-specific positive and negative controls.
• For Western blot (WB), the predicted molecular weight is 45 kDa based on the immunogen sequence; however, post-translational modifications or glycosylation may cause the endogenous protein to migrate at a different position. Validate detection conditions in the relevant sample system.
• In immunofluorescence (IF-P), because CXCR3-B is a transmembrane receptor, permeabilization with a mild detergent (e.g., 0.1% Triton X-100) may be necessary to access intracellular epitopes; optimize fixation and permeabilization protocols.
• For ELISA, pairing with a capture antibody targeting a different epitope may enhance sensitivity. Consider using recombinant protein standards for assay development.
• The antibody cross-reacts with mouse CXCR3-B, but reactivity with other species has not been tested; verify cross-reactivity if using non-human/non-mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This CXCR3-B antibody supports research and assay development in chemokine signaling, angiogenesis, and oncology, and CamelBio can assist in securing complementary reagents, custom formulations, or scale-up quantities to streamline your workflow from discovery to diagnostic application.