CXCR2 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P25025

Core Product Specifications and Parameters

Biological Background: CXCR2 Function and Localization

• CXCR2 (C-X-C chemokine receptor type 2) is a high-affinity receptor for interleukin-8 (IL-8), a potent neutrophil chemoattractant. Related references: PMID:1891716
• Ligand binding triggers G-protein mediated activation of a phosphatidylinositol-calcium second messenger cascade, leading to neutrophil activation and chemotaxis. Related references: PMID:8662698
• CXCR2 also binds with high affinity to the chemokines CXCL3, GRO/MGSA, and NAP-2, indicating a broader role in chemokine signaling beyond IL-8. Related references: PMID:1891716
• Involved in homeostatic wound healing, orchestrating neutrophil migration to injury sites while minimizing tissue damage.
• Localized at the cell membrane, consistent with its function as a G protein-coupled receptor (GPCR).
• Post-translational modifications include phosphorylation and glycosylation, as indicated by the Phosphoprotein and Glycoprotein keywords, which may regulate receptor activity and trafficking.
• Belongs to the GPCR family with seven transmembrane helices, characterized by disulfide bond formation and transducer activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain of human CXCR2 (amino acids 260–359). This region is typically less conserved across species, consistent with the reported human-specific cross-reactivity. Consider this when designing experiments with non-human samples.
• For western blot (WB), a band around 41kDa is expected. Validate the antibody with positive and negative controls in your specific sample system.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), permeabilization may be required to allow antibody access to the intracellular epitope. Optimize fixation and permeabilization conditions.
• For ELISA, the antibody can be used to capture or detect CXCR2, but pairing with another antibody may require optimization. Consider testing serial dilutions to determine optimal working concentrations.
• Given the polyclonal nature, batch-to-batch consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including this CXCR2 antibody and complementary reagents for chemokine receptor research. Our portfolio encompasses validated antibody pairs, bulk ancillary reagents, and specialized raw-material sourcing for rare targets, supporting your development from concept to clinic. Partner with CamelBio to simplify your supply chain and accelerate your IVD assay development.