CTRB1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P17538

Core Product Specifications and Parameters

Biological Background: CTRB1 Function and Localization

• CTRB1 encodes chymotrypsinogen B, the inactive zymogen precursor of the serine protease chymotrypsin, which belongs to the peptidase S1 family.
• Upon secretion into the extracellular space, proteolytic cleavage converts chymotrypsinogen B into active chymotrypsin, a key enzyme in dietary protein digestion.
• The protein contains a signal peptide for secretion and is stabilized by disulfide bonds, characteristic of serine proteases.
• Post-translational modifications include phosphorylation (as indicated by the keyword "Phosphoprotein"), and it is identified in proteomics studies of the reference human proteome.
• As a digestive serine protease, chymotrypsin B contributes to protein breakdown in the gastrointestinal tract by hydrolyzing peptide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 79–263, spanning the mature chymotrypsinogen B sequence after signal peptide cleavage; this region includes the catalytic domain and is likely to detect both the zymogen and active forms.
• For Western blot, the expected band is approximately 28 kDa under reducing conditions, but note that the zymogen (28 kDa) and mature enzyme may migrate closely.
• Consider adding protease inhibitors during sample preparation to prevent zymogen activation and degradation.
• Reactivity has been confirmed with human, mouse, and rat samples; optimize dilution and validate in your specific experimental system.
• As a polyclonal antibody, lot-to-lot consistency may vary; perform a pilot test with each new batch to ensure reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents, ensuring seamless procurement from discovery to clinical application. Our CTRB1 rabbit polyclonal antibody supports research in digestive enzyme biology and serine protease pathways, with ready-to-ship bulk formats available. Whether you need specialized antibody development or large-scale production of rare target reagents, CamelBio bridges your sourcing needs efficiently.