CTBP2 Rabbit pAb for WB, IF/ICC, ELISA - P56545

Core Product Specifications and Parameters

Biological Background: CTBP2 Function and Localization

• CTBP2 encodes C-terminal-binding protein 2, a transcriptional corepressor that interacts with diverse transcription regulators.
• It acts as a NAD-dependent oxidoreductase and plays a role in brown adipose tissue (BAT) differentiation (by similarity).
• Isoform 2 likely functions as a scaffold at specialized synapses, potentially linking transcriptional and synaptic functions.
• Subcellular localization is primarily nuclear, with additional presence at synapses, reflecting its dual role in transcription regulation and synaptic organization.
• Expression is ubiquitous, with the highest levels observed in heart, skeletal muscle, and pancreas.
• Post-translational modifications include phosphorylation and methylation, as indicated by proteomics and direct protein sequencing data.

Experimental Guidance and Technical Tips

• The immunogen targets a C-terminal peptide (amino acids 300 to the end) of human CTBP2; thus, the antibody may recognize epitopes in this region and could be suitable for detecting both full-length and C-terminal fragments.
• In Western blot, expect a band near 49 kDa corresponding to the predicted molecular weight; consider loading appropriate controls and optimizing antibody dilution.
• For IF/ICC, validate fixation and permeabilization protocols in your cell line of interest, as CTBP2 nuclear localization may require specific conditions.
• Since this is a polyclonal antibody, slight batch-to-batch variation may occur; confirm specificity using knockdown or knockout models if possible.
• Cross-reactivity with mouse and rat CTBP2 is listed; sequence homology in the immunogen region should be verified if precise species discrimination is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibodies, matched pairs, and bulk ancillary reagents. For CTBP2-related studies in transcription regulation, synapse biology, or brown adipose tissue differentiation, our supply chain expertise ensures timely access to this and other critical reagents. Whether you need custom sourcing for rare targets or scalable production of monoclonal/polyclonal antibodies, CamelBio provides a reliable bridge from concept to clinic.