CTAGE1 Rabbit pAb for WB, ELISA - Q96RT6

Core Product Specifications and Parameters

Biological Background: CTAGE1 Function and Localization

• CTAGE1, also known as cTAGE family member 2 or cancer/testis antigen 21.2 (CT21.2), is encoded by the CTAGE1 gene (also referred to as CTAGE2) in humans.
• The protein localizes to the membrane and is predicted to contain transmembrane helices, consistent with its classification as a transmembrane protein.
• Tissue expression is restricted to the testis, and it has not been detected in tumor samples, positioning it as a potential cancer/testis antigen.
• Structural features include a coiled-coil domain, which may mediate protein-protein interactions.
• Post-translational modifications include phosphorylation (as indicated by the phosphoprotein keyword), suggesting regulatory roles.
• The gene undergoes alternative splicing, generating multiple transcript variants.
• The protein has been identified through mass spectrometry-based proteomics, supporting its expression and detection in biological samples.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fragment corresponding to amino acids 370–520 of human CTAGE1 (NP_758441.2). For Western blot, expect a band around 85 kDa; consider using a blocking peptide or knockdown validation to confirm specificity.
• For ELISA applications, the recombinant immunogen can serve as a positive control or standard; cross-reactivity with mouse and rat orthologs may allow multi-species studies, but validate in the relevant sample system.
• Due to the predicted membrane localization, appropriate lysis and solubilization buffers should be considered to ensure adequate protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research into cancer/testis antigens such as CTAGE1, we offer tailored sourcing support—from rare target antibody development to reliable, scalable supplies—enabling seamless progression from concept to clinic.