CRTAP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75718

Core Product Specifications and Parameters

Biological Background: CRTAP Function and Localization

• CRTAP (Cartilage-associated protein), encoded by the CRTAP/CASP gene, is a secreted glycoprotein and member of the leprecan family.
• It is essential for the 3-hydroxylation of prolyl residues in fibrillar collagens, a critical post-translational modification ensuring collagen stability and proper extracellular matrix assembly.
• Subcellular localization is predominantly extracellular, within the extracellular matrix.
• CRTAP is highly expressed in articular chondrocytes and found across a broad range of human tissues.
• Mutations in CRTAP are associated with osteogenesis imperfecta type VII and other bone dysplasias, highlighting its role in skeletal integrity.
• Keywords from UniProt include: 3D-structure, Disease variant, Dwarfism, Extracellular matrix, Glycoprotein, Hydroxylation, Osteogenesis imperfecta, Secreted.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is ~47 kDa. Use reducing conditions and validate with appropriate positive controls from human, mouse, or rat lysates.
• In IHC-P, heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) may enhance signal; optimize antibody dilution for your tissue type.
• For ELISA, the polyclonal antibody can serve as capture or detection reagent; pair with a monoclonal antibody and perform checkerboard titration to determine optimal working concentrations.
• The immunogen corresponds to the N-terminal extracellular domain (aa 20–240), potentially suitable for both native and denatured CRTAP detection.
• As a polyclonal antibody, cross-reactivity may vary across species; validate performance in your specific sample system if using species beyond those listed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing service for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For researchers investigating collagen biology and skeletal disorders, we offer reliable CRTAP antibodies and customized procurement solutions to support your assay development. Contact us to bridge your diagnostic projects from concept to clinic.