CREM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q03060

Core Product Specifications and Parameters

Biological Background: CREM Function and Localization

• CREM is a cAMP‑responsive transcription factor that binds to cAMP response elements (CRE) in the promoters of target genes, mediating gene expression changes in response to cAMP signaling.

• Multiple CREM isoforms are generated by alternative splicing and alternative promoter usage, acting either as transcriptional activators or repressors depending on the variant.

• Essential for spermatogenesis, specifically required for spermatid maturation (PubMed:10373550). Related references: PMID:10373550

• Plays a role in circadian rhythm regulation by repressing the core clock gene PER1 through direct promoter binding.

• Highly expressed in the testes; isoform 14 is the major activator form in round spermatids.

• Localizes to both the nucleus and cytoplasm, consistent with its function in transcriptional regulation.

• Post‑translational modifications include phosphorylation and ubiquitin‑like conjugation, affecting protein stability and activity.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a primary band at approximately 37 kDa is expected based on the predicted molecular weight; however, alternative isoforms and post‑translational modifications may produce additional bands.
• In immunofluorescence/immunocytochemistry (IF/ICC), nuclear localization is typical; consider co‑staining with a nuclear marker to confirm specificity.
• Validate the antibody in relevant CREM‑expressing samples, such as testis tissue or cell lines, and include appropriate controls.
• For ELISA, optimize coating and detection conditions according to your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, supporting diagnostic manufacturers and research institutes from early development to commercialization. This CREM antibody addresses applications in transcriptional regulation, spermatogenesis, and circadian biology. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized rare‑target sourcing to accelerate your assay and kit development.