COX IV Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P13073

Core Product Specifications and Parameters

Biological Background: COX IV Function and Localization

• COX IV (Cytochrome c oxidase subunit 4 isoform 1) is encoded by the COX4I1 gene and serves as a critical nuclear-encoded subunit of mitochondrial complex IV.
• As a core component of cytochrome c oxidase, it catalyzes the final step of the electron transport chain, reducing molecular oxygen to water and driving oxidative phosphorylation.
• This enzyme contributes to the generation of the proton gradient across the inner mitochondrial membrane, essential for ATP synthesis.
• COX IV is an integral membrane protein localized to the mitochondrial inner membrane.
• Ubiquitously expressed across tissues, reflecting its fundamental role in cellular energy metabolism.
• Post-translational modifications such as acetylation and phosphorylation may modulate its activity, assembly, or stability.
• Mutations in COX4I1 are associated with primary mitochondrial disease, including cytochrome c oxidase deficiency disorders like MC4DN16.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 23–98 of human COX IV, corresponding to a region of the mature protein after mitochondrial transit peptide cleavage, which likely contributes to observed cross-reactivity with mouse and rat orthologs.
• For Western blot, anticipate a band near 20 kDa under reducing conditions; minor size variations may occur due to processing or modifications.
• In IHC-P and IF/ICC, confirm mitochondrial staining patterns and consider appropriate organelle markers for co-localization studies.
• For IP and ELISA applications, optimize antibody concentration and validate performance under native versus denaturing conditions as required by the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides this anti-COX IV polyclonal antibody and a broad portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents for mitochondrial and respiratory chain research. We support your assay development with scalable sourcing solutions, including rare-target raw materials and customized bulk supplies, backed by technical consulting from concept to clinic.