COPB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P53618

Core Product Specifications and Parameters

Biological Background: COPB1 Function and Localization

• Coatomer subunit beta (Beta-COP) is a key component of the coatomer (COPI) complex that binds dilysine motifs on cargo proteins, mediating vesicle budding from Golgi membranes.
• The coatomer complex is essential for retrograde Golgi-to-ER transport, and its recruitment in mammals depends on ADP-ribosylation factors (ARFs).
• COPB1 contributes to Golgi structural integrity, and regulates processing and endocytic recycling of LDL receptors.
• It plays a role in lipid homeostasis by modulating the localization of perilipins (PLIN2, PLIN3) and adipocyte triglyceride lipase on lipid droplets to promote lipolysis (By similarity).
• COPB1 is involved in non-canonical functions, including facilitating axonal transport and translation of kappa-type opioid receptor mRNAs.
• Subcellular localization includes cytoplasm, Golgi apparatus membrane, COPI-coated vesicle membrane, endoplasmic reticulum-Golgi intermediate compartment (ERGIC), and cell membrane.
• The protein is associated with disease phenotypes such as cataract and intellectual disability, as indicated by UniProt curation.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 460–740) of human COPB1, providing a unique epitope not conserved among other coatomer subunits; consider using this design for selective detection.
• Perform western blot analysis with a predicted band at ~107 kDa; include appropriate positive controls (e.g., HeLa, HEK293, or liver tissue lysates).
• For immunofluorescence/ICC, fix cells with paraformaldehyde and permeabilize with Triton X-100 to visualize Golgi/vesicular staining patterns.
• Validate in human, mouse, and rat samples as per cross-reactivity data; species-specific sequence alignment may help confirm epitope conservation.
• When used in ELISA, coat plates with recombinant COPB1 fragment or peptide corresponding to the immunogen for optimal binding.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material partner, CamelBio offers comprehensive access to antibodies and ancillary reagents for coatomer and intracellular trafficking research. We supply validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to accelerate your diagnostic and assay development. Whether you need COPB1 antibody cohorts or rare-target raw-material sourcing, CamelBio bridges your workflow from concept to clinic with reliable B2B support.