COP1 Rabbit Polyclonal Antibody for WB and ELISA - Q8NHY2

Core Product Specifications and Parameters

Biological Background: COP1 Function and Localization

• COP1 (Constitutive photomorphogenesis protein 1 homolog) is an E3 ubiquitin-protein ligase that mediates ubiquitination and proteasomal degradation of target proteins, including JUN, p53, 14-3-3 sigma (SFN), MTA1, and CEBPA.
• It is a RING finger and WD repeat domain-containing protein, also known as RFWD2 or RNF200, that acts as the essential RING domain subunit in certain E3 complexes, but in the DCX DET1-COP1 complex, the catalytic subunit is RBX1.
• The protein is encoded by the COP1 gene in humans (Gene ID: 64326) and is a member of the COP/DET/FUS gene family, with alternative splicing generating multiple transcript variants.
• Subcellular localization includes nuclear speckles and the cytoplasm, indicating a dynamic distribution that may reflect its diverse functions.
• COP1 plays a critical role in regulating p53 by direct ubiquitination and degradation, thereby suppressing p53-dependent transcription and apoptosis, independent of MDM2.
• Tissue expression is ubiquitous at low levels, with higher expression observed in testis, placenta, skeletal muscle, and heart.
• Key structural features include a zinc-finger domain, coiled-coil regions, and WD repeats, which mediate protein-protein interactions and substrate recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 582-731) of human COP1, suggesting that the antibody may recognize full-length COP1 and possibly isoforms containing this region.
• For Western blot (WB), consider using whole-cell lysates from human cell lines known to express COP1, such as HeLa or HEK293, and validate with molecular weight ~80 kDa.
• For ELISA, the antibody may be suitable for detecting recombinant COP1 or native protein in sample matrices, but optimization of coating and detection conditions is recommended.
• Cross-reactivity has been demonstrated for human COP1; for other species, sequence homology analysis is advised before use.
• Because COP1 functions in ubiquitination complexes, co-immunoprecipitation experiments may be possible but require validation in the relevant cellular context.

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Whether you need validated antibody pairs, optimized polyclonal antibodies like this COP1 pAb, bulk ancillary reagents, or rare target raw-material sourcing for ubiquitination and cell survival studies, CamelBio streamlines your procurement and accelerates development from concept to clinic.