Conjugated TOM20 Rabbit mAb for WB, IF/ICC, IF-P - Q15388

Core Product Specifications and Parameters

Biological Background: TOM20 Function and Localization

• TOM20 (Mitochondrial import receptor subunit TOM20 homolog), also known as MAS20 or MOM19, is a central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex.
• Together with TOMM22, it functions as a transit peptide receptor at the mitochondrial surface, facilitating preprotein translocation into the TOM40 pore. Related references: PMID:18331822
• TOM20 interacts with VDAC2 and PINK1 kinase at depolarized mitochondria, stabilizing PINK1 to trigger mitophagy via PRKN recruitment. Related references: PMID:40080546
• It is required for the translocation of cytochrome P450 monooxygenases across the mitochondrial outer membrane.
• Subcellularly, TOM20 localizes to the mitochondrial outer membrane, consistent with its role in preprotein import.
• The protein undergoes post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–145 of human TOM20, covering the cytoplasmic domain; this antibody is suitable for detecting TOM20 by Western blot (WB), immunofluorescence (IF/ICC), and fluorescence-based immunohistochemistry (IF-P).
• The antibody is directly conjugated with , enabling direct immunofluorescence detection without a secondary antibody, which may reduce non-specific binding.
• For WB analysis, consider using mitochondrial-enriched fractions from human, mouse, or rat cell lines to enhance signal specificity.
• Species cross-reactivity is reported for human, mouse, and rat; however, validate the antibody in your target species and sample type with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop procurement partner for IVD raw materials, supplying high-quality antibodies such as this anti-TOM20 conjugate for mitochondrial research and diagnostic assay development. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your workflow from concept to clinic. Contact us to discuss custom and bulk requirements tailored to your project needs.