Conjugated Anti-GFAP Rabbit Monoclonal Antibody for IF-P - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• GFAP is a class-III intermediate filament protein and a specific marker for astrocytes during central nervous system development, distinguishing them from other glial cells.
• It localizes to the cytoplasm.
• GFAP is expressed in cells lacking fibronectin.
• The protein undergoes various post-translational modifications including citrullination, methylation, and phosphorylation.
• Alternative splicing results in multiple isoforms.
• GFAP is associated with diseases such as leukodystrophy; variants in the gene can lead to pathologic conditions.
• 3D structure has been determined, and it contains a coiled-coil domain typical of intermediate filaments.
• It is a reference proteome component and has been identified by mass spectrometry.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the N-terminal region (aa 1-100) of human GFAP; consider this when designing controls and blocking experiments.
• For IF-P, consider using paraffin-embedded brain tissue sections as a positive control and ensure proper antigen retrieval techniques.
• The conjugate is photolabile; protect from light during all staining steps and storage.
• Validate cross-reactivity in mouse and rat samples if used for these species, as reactivity is reported but should be confirmed in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This GFAP antibody supports neuroscience research and diagnostic applications, and we can assist in sourcing rare targets or custom conjugations. From concept to clinic, CamelBio bridges your raw material needs with reliable supply solutions.