-conjugated Anti-CD31/PECAM1 Monoclonal Antibody for Flow Cytometry - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31/PECAM1 (Platelet endothelial cell adhesion molecule) is encoded by the Pecam1 gene and functions as a key cell adhesion molecule in vascular and immune cell interactions.
• It is essential for leukocyte transendothelial migration (TEM) under inflammatory conditions, with phosphorylation at Tyr-679 being critical for its trafficking and function (By similarity).
• PECAM1 mediates homophilic interactions contributing to endothelial cell-cell adhesion at junctions, and heterophilic binding with CD177 facilitates neutrophil transmigration (By similarity).
• Homophilic ligation on viable leukocytes transmits an anti-phagocytic "detachment" signal, whereas on apoptotic cells it promotes macrophage-mediated clearance (By similarity).
• The protein modulates bradykinin receptor BDKRB2 activation and downstream ERK1/2 signaling in response to bradykinin or hyperosmotic shock (By similarity).
• PECAM1 has been implicated in atherosclerosis susceptibility through genetic studies (PubMed:19048083). Related references: PMID:19048083
• Subcellularly, PECAM1 localizes at the cell membrane, within membrane rafts, and at cell junctions.
• Expression is prominent in endothelial cells, platelets, and certain leukocytes, with tissue detection in lung and platelets.
• Post-translational modifications include glycosylation, palmitoylation, and phosphorylation; the protein contains multiple immunoglobulin-like domains and a single transmembrane helix.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is optimized for direct flow cytometry staining. Titrate the antibody to determine the optimal concentration for your experimental system.
• Include appropriate isotype controls and viability dyes to ensure accurate gating of live cell populations.
• Fixation and permeabilization steps may affect fluorophore brightness; if intracellular staining is required, validate the protocol accordingly.
• The immunogen corresponds to an extracellular region (amino acids 18-220) of mouse CD31, ensuring specific recognition of surface-expressed PECAM1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of IVD raw materials from validated antibody pairs to bulk ancillary reagents. This anti-CD31/PECAM1 antibody supports research in endothelial biology, immunology, and vascular disease, perfectly aligning with our mission to facilitate every stage from concept to clinic. Our team can assist with custom antibody development and rare-target sourcing for your specific diagnostic needs.