-conjugated Anti-CD200/OX2 Monoclonal Antibody for Flow Cytometry - P41217

Core Product Specifications and Parameters

Biological Background: CD200/OX2 Function and Localization

• CD200 (OX-2 membrane glycoprotein) is a cell surface glycoprotein encoded by the CD200 gene (aliases MOX1, MOX2) and is a member of the immunoglobulin superfamily.
• It functions as a costimulatory molecule for T-cell proliferation and may regulate myeloid cell activity in various tissues.
• The protein is localized to the cell membrane, with a single-pass transmembrane structure, an extracellular immunoglobulin-like domain, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and the formation of a conserved disulfide bond within the immunoglobulin domain.
• Alternative splicing produces multiple isoforms, corresponding to observed molecular weights of 30 kDa, 31 kDa, and 32 kDa.
• Keywords from UniProt include alternative splicing, disulfide bond, glycoprotein, immunoglobulin domain, membrane, proteomics identification, reference proteome, signal, transmembrane, and transmembrane helix.
• CD200 plays a key role in immune tolerance and tumor immune evasion by delivering inhibitory signals through its receptor (CD200R), which is expressed on myeloid and lymphoid cells.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 31–232), ensuring recognition of the native cell surface CD200 protein, making this antibody suitable for flow cytometric analysis of live, unfixed cells.
• Since this product is directly conjugated to , it is ready to use without a secondary antibody; perform titration experiments to determine the optimal staining concentration for your specific cell type and experimental conditions.
• For intracellular staining or applications involving fixation and permeabilization, validate that the epitope is preserved, as such treatments may alter antibody binding.
• As cross-reactivity is reported only for human, researchers should verify performance when using samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a broad portfolio of IVD raw materials. For CD200/OX2-related immunoassay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your workflow from concept to clinical application. Whether you require rare target sourcing or standard reagents, our team supports your project with reliable supply and technical consulting.