Complement C3 Rabbit mAb for WB, ELISA - P01027

Core Product Specifications and Parameters

Biological Background: Complement C3 Function and Localization

• Complement C3 is a precursor protein of the complement system, giving rise to multiple fragments with distinct biological activities, including C3b and C3a.
• Also known as HSE-MSF, mouse Complement C3 plays a central role in innate immunity and inflammatory responses.
• As C3b, it acts as an opsonin by covalently attaching to pathogen surfaces via a reactive thioester bond, marking them for phagocytosis.
• C3b forms part of the C5 convertase in both the classical/lectin (with C4b and C2) and alternative (with CFB) pathways, driving downstream complement activation.
• The C3a anaphylatoxin fragment is a potent pro-inflammatory mediator, binding to the C3AR1 receptor to trigger mast cell degranulation, histamine release, and chemotaxis.
• Beyond immunity, C3 exhibits adipogenic functions, stimulating triglyceride synthesis and glucose transport in adipocytes through C5AR2 receptor signaling.
• The full-length protein has a molecular weight of approximately 186 kDa and is primarily secreted, with localization also observed on cell surfaces.
• Key UniProt keywords include Complement pathway, Innate immunity, Inflammatory response, and Fatty acid metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1364–1663) of mouse Complement C3, which may contribute to the observed cross-reactivity with rat.
• For Western blotting, the predicted full-length band is ~186 kDa; cleavage fragments (e.g., C3b, C3a) may appear under activating conditions. Positive controls (mouse serum or stimulated cell lysates) are recommended.
• For ELISA, consider optimizing pairing with a matched antibody and validate in the relevant sample matrix.
• Since C3 is an acute-phase protein, its expression can be upregulated by inflammatory stimuli; consider using stimulated samples or inflammatory models for enhanced detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with seamless access to validated IVD raw materials, including monoclonal antibodies like this Complement C3 Rabbit mAb for immunoassay development. Our portfolio extends to optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—supporting your complement pathway and innate immunity research from concept to clinic.