Collagen IV Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q01955 / P29400 / P08572 / P02462

Core Product Specifications and Parameters

Biological Background: Collagen IV (COL4A3) Function and Localization

• Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans, and entactin/nidogen.
• Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
• COL4A3 is secreted and localized to the extracellular space and basement membrane.
• Alpha 3 and alpha 4 type IV collagens are colocalized in kidney, eye, lens capsule, cochlea, lung, skeletal muscle, and fetal tissues. Expression levels vary by tissue and isoform. Related references: PMID:8083201 PMID:7523402
• Mutations in COL4A3 cause Alport syndrome, characterized by progressive kidney disease and hearing loss, highlighting its critical role in basement membrane integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1555–1669 of human collagen alpha-1(IV), which may confer cross-reactivity with multiple type IV collagen alpha chains.
• For Western blot, anticipate bands around 160–168 kDa, corresponding to different alpha chains; use appropriate positive controls (e.g., human kidney or lung lysates).
• For IF/ICC, optimize fixation and permeabilization conditions to visualize basement membrane structures; co-staining with other basement membrane markers is recommended.
• Validate the antibody in the relevant sample system, as performance may vary across applications and sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive one-stop platform for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This Collagen IV rabbit monoclonal antibody supports basement membrane studies and diagnostic assay development. Let CamelBio streamline your supply chain and accelerate your project from concept to clinic.