COLGALT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NBJ5

Core Product Specifications and Parameters

Biological Background: COLGALT1 Function and Localization

• COLGALT1 (Procollagen galactosyltransferase 1), also known as GLT25D1, BSVD3, or ColGalT 1, is a member of the glycosyltransferase 25 family.
• It functions as a beta-galactosyltransferase that specifically transfers beta-galactose to hydroxylysine residues of type I collagen.
  Related references: PMID:19075007 PMID:22216269 PMID:27402836
• By glycosylating collagen, COLGALT1 facilitates the formation of the collagen triple helix, a key step in collagen maturation.
  Related references: PMID:27402836
• It is also involved in the biosynthesis of collagen type IV, contributing to basement membrane integrity.
  Related references: PMID:30412317
• The enzyme localizes to the endoplasmic reticulum lumen, where post-translational modification of procollagen takes place.
• COLGALT1 is ubiquitously expressed with elevated levels in placenta, heart, lung, and spleen.
• Mutations in COLGALT1 are linked to disease variants, highlighting its essential role in collagen-related disorders.

Experimental Guidance and Technical Tips

• For Western blot (WB), use the predicted molecular weight of 72 kDa as a reference; validate in cell or tissue lysates known to express COLGALT1.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization to preserve endoplasmic reticulum morphology and antibody accessibility.
• For ELISA applications, consider using recombinant COLGALT1 protein or collagen substrates for assay development.
• When employing mouse or rat models, confirm antibody cross-reactivity experimentally due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our COLGALT1 polyclonal antibody supports research into collagen glycosylation and triple helix formation, with complementary products available for glycosylation pathway and extracellular matrix studies. We assist diagnostic manufacturers and laboratories in efficiently bridging from concept to clinical application.