COL9A3 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q14050

Core Product Specifications and Parameters

Biological Background: COL9A3 Function and Localization

• Collagen alpha-3(IX) chain, encoded by the COL9A3 gene, belongs to the fibril-associated collagens with interrupted triple helices (FACIT) family.
• It acts as a structural component of hyaline cartilage and the vitreous humor of the eye, contributing to tissue integrity and transparency.
• The protein is secreted and localizes to the extracellular matrix, where it interacts with other collagen types to organize the supramolecular architecture.
• COL9A3 is implicated in Stickler syndrome, a genetic disorder characterized by hearing loss, dwarfism, and ocular abnormalities, as well as in multiple epiphyseal dysplasia.
• Post-translational modifications include hydroxylation of proline and lysine residues and glycosylation, essential for collagen stability and function.
• Experimental 3D structures have been determined, providing insights into its molecular assembly and potential binding interfaces.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 445-684, a region that may include the collagenous domain; this design can enhance specificity for the target in denaturing or native conditions.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), consider antigen retrieval protocols and validate staining in cartilage or ocular tissues known to express COL9A3.
• Cross-reactivity with mouse is reported; this antibody may be suitable for translational studies using mouse models, though independent validation is advised.
• ELISA performance can be optimized by using recombinant COL9A3 protein as standard; check for matrix effects in complex samples.
• As a polyclonal antibody, lot-to-lot consistency should be monitored by Western blot or other application-specific assays if quantitative comparisons are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. This COL9A3 Rabbit pAb supports investigations in skeletal dysplasias and ocular pathologies, aligning with our supply network for collagen-related antigens and assay development. Leverage our integrated procurement services to accelerate your project from concept to clinic.