COL9A2 Rabbit Polyclonal Antibody for WB, ELISA - Q14055

Core Product Specifications and Parameters

Biological Background: COL9A2 Function and Localization

• Collagen alpha-2(IX) chain, encoded by the COL9A2 gene, is a structural component of hyaline cartilage and the vitreous of the eye.
• It is a secreted extracellular matrix protein, located in the extracellular space.
• The protein belongs to the collagen family and features repeat regions, disulfide bonds, and post-translational modifications including glycosylation and hydroxylation.
• UniProt keywords associate COL9A2 with deafness, dwarfism, Stickler syndrome, and other disease variants.
• The mature protein has a molecular weight of approximately 65 kDa (UniProt: 65131 Da).

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 600-689) of human COL9A2 (NP_001843.1), which may recognize both full-length and processed forms. Validate band specificity in your sample system.
• For WB, consider using reducing conditions and a positive control such as cartilage tissue lysate; expected band size ~65 kDa.
• In ELISA, the immunizing peptide can serve as an antigen standard to assess antibody reactivity.
• Cross-reactivity with rat COL9A2 is reported; verify in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and researchers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. The COL9A2 Rabbit Polyclonal Antibody aids in the study of collagen-related extracellular matrix targets, particularly in cartilage and vitreous research. Explore our comprehensive portfolio for rare target sourcing and assay development.